Generation of renal tubular organoids from adult SOX9⁺ kidney progenitor cells.

ABSTRACT: The pathogenesis of several kidney diseases results in the eventual destruction of the renal tubular system, which can progress to end-stage renal disease. Previous studies have demonstrated the involvement of a population of SOX9-positive cells in kidney regeneration and repair process following kidney injury. However, the ability of these cells to autonomously generate kidney organoids has never been investigated. Here, we isolated SOX9⁺ kidney progenitor cells (KPCs) from both mice and humans and tested their differentiation potential in vitro. The data showed that the human SOX9⁺ KPC could self-assemble into organoids with kidney-like morphology. We also used single-cell RNA sequencing to characterize the organoid cell populations and identified four distin

SUBMITTER: Zhou D 

PROVIDER: S-EPMC11749593 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature