Systematic analysis of the glucose-PTS in <i>Streptococcus sanguinis</i> highlighted its importance in central metabolism and bacterial fitness.

ABSTRACT: Previous work reported that deletion of the Enzyme IIAB subunits (EIIAB^Man and manL) of the glucose phosphotransferase system (PTS) (glucose-PTS, manLMNO) in Streptococcus sanguinis impacted carbon catabolite repression and bacterial fitness. Here, a single-nucleotide polymorphism in ManN, ManNA91E, produced the unusual phenotype of increased excretion of organic acids and H₂O₂ yet elevated PTS activities. To characterize the contributions of each component of the glucose-PTS to bacterial fitness, we performed genetic analyses by deleting from S. sanguinis SK36 the entire operon and each EII^Man subunit individually; and genes encoding the catabolite control protein A (ΔccpA) and the redox regulator Rex (Δrex<

SUBMITTER: Taylor ZA 

PROVIDER: S-EPMC11784104 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature