Radio-sensitizing effect of MEK inhibition in glioblastoma in vitro and in vivo.

ABSTRACT:

Introduction

Glioblastoma (GBM) is an incurable cancer type. New therapeutic options are investigated, including targeting the mitogen-activated protein kinase (MAPK) pathway using MEK inhibitors as radio-sensitizers. In this study, we investigated whether MEK inhibition via PD0325901 leads to radio-sensitization in experimental in vitro and in vivo models of GBM.

Materials and methods

In vitro, GBM8 multicellular spheroids were irradiated with 3 fractions of 2 Gy, during 5 consecutive days of incubation with either PD0325901 or MEK-162. In vivo, we combined PD0325901 with radiotherapy in the GBM8 orthotopic mouse model, tumor growth was measured weekly by bioluminescence imaging and overall survival and toxicity were assessed.

Results

Regrowth and viability of sphero

SUBMITTER: Houweling M 

PROVIDER: S-EPMC11798055 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature