Infection pattern of male-killing viruses alters phenotypes in the tea tortrix moth Homona magnanima.

ABSTRACT: Male-killing is a microbe-induced reproductive manipulation in invertebrates whereby male hosts are eliminated during development. In the tea tortrix moth Homona magnanima, Osugoroshi viruses 1‒3 (OGVs), belonging to Partitiviridae induce male-killing. The infection patterns of OGVs are diverse; however, how the influence of these patterns of host phenotypes remains largely unknown. Using field-collected larvae, we established a OGV1 and OGV3 double-infection line, in addition to a triple-infection line, and examined the dsRNA segments, purified viral proteins, OGV density, and host phenotypes. PCR analysis demonstrated that the triple-infection line lost one dsRNA segment, whereas the double-infection line lost eight segments, including one RNA-dependent RNA polymerase (RdRp) gene. LC-MS

SUBMITTER: Takamatsu T 

PROVIDER: S-EPMC11799345 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature