Comparison of chromatin accessibility remodeling of granulosa cells in patients with endometrioma or pelvic/tubal infertility.

ABSTRACT:

Purpose

To elucidatethe epigenetic alteration associated with impaired oogenesis in endometrioma using multi-omic approaches.

Methods

ATAC-seq was performed on the granulosa cells (GCs) of 6 patients (3 with endometrioma and 3 without). Follicular samples from another 20 patients (10 with endometrioma and 10 without) were collected for mRNA-seq analysis of GCs and extracellular vesicles (EVs) of follicular fluid. qRT-PCR validated candidate genes in GCs from 44 newly enrolled patients (19 with endometrioma and 25 without). mRNA abundance was compared with the Mann-Whitney test. Pearson's correlation analyzed relationships between candidate genes and oocyte parameters.

Results

Chromatin accessibility and gene expression profiles of GCs from endometrioma patients differ

SUBMITTER: Ou S 

PROVIDER: S-EPMC11871191 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature