Aberrant DNA methylation of genes regulating CD4+ T cell HIV-1 reservoir in women with HIV.

ABSTRACT:

Background

The HIV-1 reservoir in CD4+ T cells (HR_CD4) pose a major challenge to curing HIV, with many of its mechanisms still unclear. HIV-1 DNA integration and immune responses may alter the host's epigenetic landscape, potentially silencing HIV-1 replication.

Methods

This study used bisulphite capture DNA methylation sequencing in CD4+ T cells from the blood of 427 virally suppressed women with HIV to identify differentially methylated sites and regions associated with HR_CD4.

Results

The average total HR_CD4 size was 1409 copies per million cells, with most proviruses defective and only a small proportion intact. The study identified 245 differentially methylated CpG sites and 85 regions linked to HR_CD4 size, with 52% of s

SUBMITTER: Xu K 

PROVIDER: S-EPMC11896887 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature