Early onset of septal FtsK localization allows for efficient DNA segregation in SMC-deleted <i>Corynebacterium glutamicum</i> strains.

ABSTRACT: Structural maintenance of chromosomes (SMC) are ubiquitously distributed proteins involved in chromosome organization. Deletion of smc causes severe growth phenotypes in many organisms. Surprisingly, smc can be deleted in Corynebacterium glutamicum, a member of the Actinomycetota phylum, without any apparent growth phenotype. SMC in C. glutamicum is loaded in a ParB-dependent fashion to the chromosome and functions in replichore cohesion. The unexpected absence of a growth phenotype in the smc mutant prompted us to screen for synthetic interactions within C. glutamicum. We generated a high-density Tn5 library from wild-type and smc-deleted C. glutamicum strains. Transposon sequencing data revealed that the DNA translocase FtsK

SUBMITTER: Peng F 

PROVIDER: S-EPMC11898615 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature