Potential role of liver-resident CD3⁺ macrophages in HBV clearance in a mouse hepatitis B model.

ABSTRACT:

Background & aims

Chronic HBV infection usually causes cirrhosis and hepatocellular carcinoma. Comparative investigations of acute and chronic HBV cases would help determine the immune responses crucial for viral clearance.

Methods

A fast-cleared HBV mouse model was established in Alb-Cre mice via hydrodynamic injection of HBV plasmid, while persistent HBV model mice were generated via recombinant covalently closed circular DNA-adeno-associated virus 8 infection. The single-cell transcriptomes of CD45⁺ intrahepatic non-parenchymal cells from these mice were conducted. Multiplexed immunohistochemistry and flow cytometry were used to confirm the findings from single-cell transcriptomes. Transwell, coculture, and adoptive transfer experiments were performed to study

SUBMITTER: Wang C 

PROVIDER: S-EPMC11937660 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature