Chemical reprogramming culture for the expansion of salivary gland epithelial basal progenitor cells.

ABSTRACT:

Background

Salivary gland (SG) hypofunction presents a significant clinical challenge with limited treatment options. SG epithelial cells offer a promising approach due to their intrinsic tissue specificity and regenerative potential. However, the lack of efficient culture methods has hindered their clinical use.

Methods

This study presents a chemical reprogramming culture (CRC) system that utilizes a combination of three small molecules for the long-term two-dimensional culture of human SG epithelial progenitor cells. We characterized the cultured cells, measured their organoid-forming efficiencies, and assessed their differentiation potential. To evaluate the therapeutic efficacy of the SG basal progenitor cells (SG-BPCs), we administered them into a mouse model with radia

SUBMITTER: Jeong YJ 

PROVIDER: S-EPMC12008940 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature