Quantitative Changes in the Proteome of Chronically Inflamed Lacrimal Glands From a Sjogren's Disease Animal Model.

ABSTRACT:

Purpose

The lacrimal gland (LG) is the major source of aqueous tears, and insufficient LG secretion leads to aqueous-deficient dry eye (ADDE) disease. To provide a foundational description of LG's protein expression patterns, we prepared protein extracts of LGs from a wild-type and an ADDE mouse model and analyzed the proteome by quantitative mass spectrometry.

Methods

LGs were isolated from an ADDE mouse model, male non-obese diabetic (NOD) mice and control wild-type BALB/c mice (n = 6 each). Protein samples were prepared in urea-based lysis buffer and protein concentrations determined by the BCA method. The equivalent of 200 µg protein were tryptically digested and analyzed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS). Proteins were identified and

SUBMITTER: Toribio D 

PROVIDER: S-EPMC12013672 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature