Whole pulse ingredient inclusion in macronutrient-balanced diets increased fecal concentrations of propionic acid but not total bile acids in healthy adult large-breed dogs after 20 weeks.

ABSTRACT: This study investigated the effects of up to 45% inclusion of whole pulse ingredients in grain-free (GF) diets on the excretion of bile acids (BAs) and other fecal metabolites in healthy large-breed dogs. Twenty-eight adult Siberian Huskies were fed 1 of 4 experimental diets formulated to meet the same macronutrient profiles for 20 wk: 1) grain-inclusive diet with 45% corn (Ctl), 2) GF diet with 15% pulses (Pulse15), 3) GF diet with 30% pulses (Pulse30), 4) GF diet with 45% pulses (Pulse45). All diets included chicken meal and pea starch. Fecal samples were collected on weeks 2 and 19. Bile acids were analyzed using ultra-performance liquid chromatography-MRM/MS technology, while fecal metabolites were analyzed using Agilent HP1000 high-performance liquid chromatography. Bile acids and fec

SUBMITTER: Singh P 

PROVIDER: S-EPMC12019969 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature