Development of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air.

ABSTRACT: Several studies have successfully detected SARS-CoV-2 in air samples. However, most of these studies focused on validating the air collection method, and there was no report on the development of a virus detection method. In this study, to detect viruses in air samples with greater sensitively than conventional detection methods, we utilized COPMAN, a highly sensitive virus detection method originally used for wastewater samples. We applied COPMAN to air samples, thereby developing COPMAN-Air. Briefly, this method efficiently detects the extremely low levels of viral RNA in air samples via three reaction steps: RT, preamplification, and qPCR, as it is performed with COPMAN. We evaluated COPMAN-Air using samples from a fever clinic for COVID-19 patients. COPMAN-Air demonstrated a higher det

SUBMITTER: Yoshinaga T 

PROVIDER: S-EPMC12022069 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature