Decoding G-Quadruplexes Sequence in <i>Vitis vinifera</i>: Regulatory Region Enrichment, Drought Stress Adaptation, and Sugar-Acid Metabolism Modulation.

ABSTRACT: G-quadruplexes play a crucial role in transcription, translation, and DNA replication in plant genomes. Here, we comprehensively examined the prevalence and functions of G-quadruplexes in Vitis vinifera. A total of 467,813 G-quadruplexes were identified in grapevine genome, with enrichment in the promoter (0.54/kbp) and near transcription start sites (TSSs, 1.00/kbp), and showed conservative strand preference. The G-quadruplex density in centromeres exhibited heterogeneity. The differentially expressed genes (DEGs) under two-day drought stress manifested high G-quadruplex density in the promoter and TSS regions. The upregulated DEGs showed template strand-biased G-quadruplex enrichment, while downregulated DEGs displayed coding strand dominance linked to metal ion homeostasis and su

SUBMITTER: Xie J 

PROVIDER: S-EPMC12030360 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature