Direct genome-scale screening of Gluconobacter oxydans B58 for rare earth element bioleaching.

ABSTRACT: The transition to a sustainable energy economy will require an enormous increase in the supply of rare earth elements (REEs). Bioleaching offers a promising alternative to conventional hydrometallurgical methods for REE extraction from low-grade ores. However, exploiting this potential remains challenging due to large gaps in our understanding of the genetics involved, and inadequate biological tools to address them. We generated a highly non-redundant whole-genome knockout collection for the bioleaching microbe Gluconobacter oxydans B58, reducing redundancy by 85% compared to the previous best collection. This new collection was directly screened for bioleaching neodymium from a synthetic monazite powder, identifying 89 genes important for bioleaching, 68 of which have not previously been

SUBMITTER: Marecos S 

PROVIDER: S-EPMC12041372 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature