Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations.

ABSTRACT: Caenorhabditis elegans (C. elegans) has been and remains a valuable model organism to study developmental biology, aging, neurobiology, and genetics. The large body of work on C. elegans makes it an ideal candidate to integrate into large-population, whole-animal studies to dissect the complex biological components and their relationships with another organism. In order to use C. elegans in collaborative -omics research, a method is needed to generate large populations of animals where a single sample can be split and assayed across diverse platforms for comparative analyses. Here, a method to culture and collect an abundant mixed-stage C. elegans population on a large-scale culture plate (LSCP) and subsequent phenotypic data is presented. This pipeline yields sufficient numbers of animals

SUBMITTER: Shaver AO 

PROVIDER: S-EPMC12042146 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature