Comprehensive sequencing profile and functional analysis of IsomiRs in human pancreatic islets and beta cells.

ABSTRACT:

Aims/hypothesis

MiRNAs regulate gene expression, influencing beta cell function and pathways. Isoforms of miRNA (isomiRs), sequence variants of miRNAs with post-transcriptional modifications, exhibit cell-type-specific expression and functions. Despite their biological significance, a comprehensive isomiR profile in human pancreatic islets and beta cells remains unexplored. This study aims to profile isomiR expression in four beta cell sources: (1) laser capture microdissected human islets (LCM-HI); (2) collagenase-isolated human islets (CI-HI); (3) sorted beta cells; and (4) the EndoC-βH1 beta cell line, and to investigate their potential role in beta cell function.

Methods

Small RNA-seq and/or small RNA dataset analysis was conducted on human pancreatic islets and beta cel

SUBMITTER: Auddino S 

PROVIDER: S-EPMC12069488 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature