<i>Microaceticoccus formicicus</i> gen. nov., sp. nov., an ammonia-tolerant formate-utilizing bacterium originating from a biogas process.

ABSTRACT: A strictly anaerobic bacterial strain, designated as AMB_02^T, was isolated from a propionate enrichment culture obtained from a high-ammonia biogas digester. The cells were anaerobic and coccoid (0.5 µm), often appearing as diplococci or in a short chain of three to four cells. Growth was observed between 20 and 45 °C (optimum at 37-39 °C), with an initial pH of 6.5-9.0 (optimum pH 8.0-8.5), and the species tolerated up to 0.8 M NH₄Cl and 0.5 M NaCl. The major cellular fatty acids were C_16 : 0 (31.6%), C_14 : 0 (14.6%) and C_18 : 0 (13.3%). AMB_02^T grew with formate, carbohydrates and aa, including asparagine, histidine, tryptone and tryptophan. Acetate was the major product formed. Phylogenetic analysis based on 16S rRNA gene

SUBMITTER: Cheng G 

PROVIDER: S-EPMC12079837 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature