Development and Application of a TaqMan-Based qPCR Assay for Detecting ENTV-2 in Goats.

ABSTRACT:

Background

In recent years, enzootic nasal tumor virus 2 (ENTV-2) has become prevalent in China, resulting in substantial economic losses for the goat industry. In order to enrich the availability of detection methods for ENTV-2, this study developed an expedited and accurate reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) assay to facilitate the detection and quantification of ENTV-2.

Methods

Specifically, a pair of primers and a TaqMan probe targeting conserved regions of the pro gene were designed to allow the specific amplification and detection of viral RNA in clinical samples. Moreover, modifying the method for use in a quantitative real-time PCR (qPCR) assay enables the detection of proviral DNA in tumor specimens.

Results

SUBMITTER: Li P 

PROVIDER: S-EPMC12111429 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature