Oxaloacetate and Ketone Bodies Synergistically Promote Myoblast Differentiation in L6 Cells.

ABSTRACT: Malnutrition and aging are major factors that inhibit myoblast differentiation, leading to a decline in muscle function and contributing to sarcopenia development. This study aimed to elucidate the role of nutrients in myoblast differentiation by establishing a culture system at physiological glucose levels and investigating the effects of ketone bodies and oxaloacetate. We successfully cultured myoblasts at physiological glucose concentrations in a hydrophobic membrane filter-equipped culture flask. Under these conditions, ketone bodies and oxaloacetate synergistically upregulated myogenic differentiation markers (Lmod2 and Ckm), indicating enhanced differentiation. Additionally, oxaloacetate upregulated mitochondrial biogenesis markers (mitochondrial DNA copy number and Cs)

SUBMITTER: Onuki Y 

PROVIDER: S-EPMC12114476 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature