The HL-60 clone 15 cell line as a model for leukocyte migration-possibilities and limitations.

ABSTRACT:

Introduction

As a part of the innate immune system, eosinophils are recruited during infectious diseases, to release their characteristic cytotoxic granules and catch pathogens in extracellular traps. Moreover, eosinophils have a crucial role in autoimmune diseases, for example allergies. The isolation of these densest and lowest abundant leukocytes is cost-and labor intense. This sets restrictions on many aspects of eosinophilic research. In this study, we performed a thorough characterization and functional assessment of the HL-60 clone 15 (HC15) cell line, which can be differentiated into eosinophil-like cells, to investigate its potential in eosinophil research.

Methods

HC15 cells were differentiated with sodium butyrate with or without IL-5 and cells were characterized

SUBMITTER: Griesbaum L 

PROVIDER: S-EPMC12148902 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature