Selection of reference genes for real-time PCR in human hepatocellular carcinoma tissues.

ABSTRACT:

Aims

To investigate the most suitable housekeeping genes for quantifying a change in mRNA expression levels due to hepatitis virus B related hepatocellular carcinoma (HCC).

Methods

Expression of mRNA encoding ACTB, GAPDH, B2M, HPRT and TBP was measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in matched malignant and non-malignant tissues obtained from 65 non-treated HCCs. The software programs geNorm and NormFinder were used to ascertain the most suitable reference gene combination.

Results

All candidate genes showed significantly different expression between malignant and non-malignant samples. GAPDH and ACTB, genes most frequently used for normalization, were heavily regulated during HCC carcinogenesis and progression. B2M expres

SUBMITTER: Gao Q 

PROVIDER: S-EPMC12160763 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature