Rapid and sensitive detection of <i>Mycobacterium tuberculosis</i> using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts.

ABSTRACT: Tuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 Trans Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen detection, was constrained by its reliance on specialized fluorescence equipment and lack of visual output, limiting its use in resource-limited settings. To overcome these limitations, we introduce an innovative RPA/CRISPR-Cas12f1_ge4.1 dual-mode system for rapid Mycobacterium tuberculosis detection. This system combines engineered Cas12f_ge4.1 with recombinase polymerase amplification (RPA), offering both fluorescent and lateral flow detection. It achieves high sensitivity with detection li

SUBMITTER: Deng Z 

PROVIDER: S-EPMC12211024 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature