Nucleobase self-assembly: aggregation, morphological characterization, and toxicity analysis.

ABSTRACT: This study expands the platform for amyloidogenic building blocks, such as nucleobases, and their self-assembly. Here, we examine the self-assembly profile of nucleobases such as guanine, cytosine, and thymine and determine that these nucleobases, while aged, produce small globules which gradually transform into fibrillar assemblies. Notably, the amyloid-like fibrillation in adenine and uracil has already been reported; hence, it was imperative to understand the amyloidogenic propensity in these unexplored nucleobases. The aggregates formed by guanine, cytosine, and thymine interestingly reveal a distinctive spectrum characteristic of amyloidogenic proteins after binding to the amyloid-specific dye Thioflavin T (ThT). The MTT assay in human retinal pigment epithelial RPE-1 cell lines revea

SUBMITTER: Dave R 

PROVIDER: S-EPMC12258404 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature