Antioxidant and Anti-inflammatory Activity of Eugenol, Bis-eugenol, and Clove Essential Oil: An In Vitro Study.

ABSTRACT: Different therapeutic approaches, particularly those involving plant-derived compounds, have been explored for treating inflammatory diseases. This study evaluated the antioxidant and anti-inflammatory properties of eugenol (EU) in its pure form, bis-eugenol (BIS), and clove essential oil (OE) at different concentrations (5, 10, and 25 μg/mL). Chemical analysis confirmed that OE contains a high proportion of eugenol (45-90%), with the presence of eugenol acetate differentiating it from pure eugenol, while bis-eugenol was successfully synthesized and characterized with a 97% yield. Antioxidant activity assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed that 25 μg/mL of each compound effectively neutralized free radicals and reduced fe

SUBMITTER: Pires Costa E 

PROVIDER: S-EPMC12290672 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature