Project description:A comprehensive systemic assessment of the proteome changes that occur in prostate cancer (PCa) during the onset and course of the illness may produce results that are clinically significant. Proteins, the main participants in the majority of biological events, are both potential biomarkers and therapeutic targets. Although the PCa tissue proteome has already been described, screening tissue samples is an intrusive operation. As a result, we concentrated on liquid biopsies like urine samples for this investigation. Specifically, liquid chromatography coupled to high-resolution mass spectrometry (LC-MS/MS) was employed to examine the urinary small extracellular vesicle (EV) proteome patterns of 100 patients. The main objective of this study is to identify biomarkers for PCa patients with cribriform pattern and intraductal PCa (IDC).

2025-05-06 | PXD043874 | Pride

Urinary extracellular vesicle-derived miR-126-3p predicts lymph node invasion in patients with high-risk prostate cancer.

Project description: Not available

| S-EPMC11153291 | biostudies-literature

A Model to Detect Significant Prostate Cancer Integrating Urinary Peptide and Extracellular Vesicle RNA Data.

Project description: Not available

| S-EPMC9027643 | biostudies-literature

Plasma Preparation Strategies for Extracellular Vesicle-Based Biomarkers in Metastatic Castration-Resistant Prostate Cancer.

Project description: Not available

| S-EPMC12311838 | biostudies-literature

Extracellular vesicle protein and miRNA signatures as biomarkers for post-infectious ME/CFS patients

Project description:Post-infectious Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a chronic disease with a yet unresolved pathophysiology and sparse diagnostic options. Extracellular vesicles (EVs) serve as carriers of disease-specific protein and microRNA (miRNA) signatures. We aim to identify novel miRNA markers as potential biomarker in isolated plasma EVs from female post-COVID-19 ME/CFS patients in comparison to healthy controls (HCs). EVs were isolated from plasma of post-infectious ME/CFS patients and HCs by size exclusion chromatography, and characterized for their number, size, morphology and surface marker expression. Small RNA sequencing identifed four small RNAs, which are significantly differential expressed between ME/CFS patients and healthy donors. Subsequently performed qPCR studies revealed significant downregulation exclusively for the hsa-let-7b-5p miRNA in EVs from ME/CFS patients versus HCs. Notably, reduced hsa-let-7b-5p expression correlated with impaired physical functioning as well as increased fatigue, pain, and immune activation scores. Our data indicate, that hsa-let-7b-5p could serve as a promising candidate for development as diagnostic and stratification marker of ME/CFS.

2026-03-18 | GSE317067 | GEO

Bladder cancer detection by urinary extracellular vesicle mRNA analysis.

Project description: Not available

| S-EPMC6132352 | biostudies-literature