Knockout of <i>GmCKX3</i> Enhances Soybean Seed Yield via Cytokinin-Mediated Cell Expansion and Lipid Accumulation.

ABSTRACT: Soybean is a dual-purpose crop for food and oil, playing a crucial role in China's grain production. Seed size and weight are key agronomic traits directly influencing the yield. Cytokinin oxidases/dehydrogenases (CKXs) specifically degrade certain isoforms of endogenous cytokinins (CKs), thereby modulating plant growth and seed development. However, their role in soybeans remains largely uncharacterized. In a previous genome-wide association study of 250 soybean core germplasms, we identified GmCKX3 as a yield-related gene. To elucidate its function, we developed GmCKX3-deficient mutants using CRISPR/Cas9 gene editing in soybean Williams82 and conducted a three-year phenotypic analysis. Loss of GmCKX3 function significantly enhanced the seed size and weight, which was

SUBMITTER: Li X 

PROVIDER: S-EPMC12299880 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature