Influence of Peptide Conjugation Sites on Lunatin-Alumina Nanoparticles: Implications for Membrane Interaction and Antimicrobial Activity.

ABSTRACT: Background/Objectives: The increasing prevalence of multidrug-resistant bacteria presents a major global health challenge, prompting a search for innovative antimicrobial strategies. This study aimed to develop and evaluate a novel nanobiostructure combining alumina nanoparticles (NPs) with the antimicrobial peptide lunatin-1 (Lun-1), forming peptide-functionalized nanofilaments. The main objective was to investigate how the site of peptide functionalization (C-terminal vs. N-terminal) affects membrane interactions and antibacterial activity. Methods: NP-peptide conjugates were synthesized via covalent bonding between lun-1 and alumina NP and characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), zeta potential analysis, dynamic light scattering

SUBMITTER: Ferreira CS 

PROVIDER: S-EPMC12299903 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature