Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein.

ABSTRACT:

Background

African swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.

Results

In this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D,

SUBMITTER: He J 

PROVIDER: S-EPMC12326869 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature