Genome Mining-Based Discovery of Pyrano[2,3-<i>c</i>]pyrrole Type Natural Products Possessing Alkyl Side Chain with Branched Methyl Groups.

ABSTRACT: Genome mining of polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) in fungal endophyte Neopestalotiopsis olumideae strain BRIP 39872 identified seven new natural products (olumilides A-D, D1, D1', D2) with pyrano[2,3-c]pyrrole scaffolds and their biosynthetic precursor preolumilide. Unlike known congeners, such as curvupallides, olumilides possess alkyl side chains with branched methyl groups and threonine-derived core scaffolds. Absolute configurations were determined by microED and ECD simulations, providing valuable information for further research focusing on the stereochemistry of PKS-NRPS reactions.

SUBMITTER: Shi Y 

PROVIDER: S-EPMC12340962 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature