Molecular Insights into the Superiority of Platelet Lysate over FBS for hASC Expansion and Wound Healing.

ABSTRACT: Human adipose-derived stem cells (hASCs) are widely used in regenerative medicine due to their accessibility and high proliferative capacity. Platelet lysate (PL) has recently emerged as a promising alternative to fetal bovine serum (FBS), offering superior cell expansion potential; however, the molecular basis for its efficacy remains insufficiently elucidated. In this study, we performed RNA sequencing to compare hASCs cultured with PL or FBS, revealing a significant upregulation of genes related to stress response and cell proliferation under PL conditions. These findings were validated by RT-qPCR and supported by functional assays demonstrating enhanced cellular resilience to oxidative and genotoxic stress, reduced doxorubicin-induced senescence, and improved antiapoptotic properties.

SUBMITTER: Kunieda S 

PROVIDER: S-EPMC12346565 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature