Exploring the significance of palmitoylation using an artificial protein lipidation system.

ABSTRACT: Protein lipidation, particularly palmitoylation (attachment of a 16-carbon fatty acid), regulates cellular behaviors by controlling protein function at lipid membranes. In this study, we prepared a series of lipidated green fluorescent proteins ("EGFP-lipids") with various alkyl chain lengths (C8 to C22). Using model lipid membranes and Jurkat (human T lymphocyte) cells, we evaluated how lipidation affects the membrane interactions and vesicular transport from the membrane of these protein-lipid constructs. Our findings demonstrate that elongation of the alkyl chain profoundly affects both lateral membrane diffusion and vesicular transport of the EGFP-lipids. Only artificially lipidated proteins that mimic in vivo lipidation exhibited cellular dynamics in response to external signal

SUBMITTER: Uchida K 

PROVIDER: S-EPMC12351397 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature