Unraveling the Effects of Epigallocatechin-3-gallate on Hepatocellular Carcinoma Cells: A Comparative Analysis of Monolayer vs Multicellular Tumor Spheroids.

ABSTRACT: The tumor microenvironment is a complex milieu that has not been properly studied in cells cultured in conventional monolayer. Studies have demonstrated the antitumor activity of epigallocatechin-3-gallate (EGCG), present in green tea, using monolayer cultures without considering the three-dimensional microenvironment of a tumor. Furthermore, many studies have shown the effect of EGCG on the transcriptional profile of cancer cells, but each study has been limited to only one or a few cell types. Using the LINCS database, we characterized the gene signatures produced by EGCG treatment in different cell types and reported a variation in EGCG-induced gene signatures depending on the cell type analyzed. GSEA analysis revealed that EGCG influenced multiple biological pathways related to cell si

SUBMITTER: Dos Reis Simpronio M 

PROVIDER: S-EPMC12355261 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature