Targeted genomic analysis of a predominant uncultured marine pelagiphage-host model via microfluidics and semipermeable capsule technology.

ABSTRACT: Microbes and their viruses drive central biogeochemical cycles on a global scale. Understanding the biology and ecology of virus-host interactions and their impact on ecosystems depends on our ability to develop tools that enable high-throughput screening of ecologically relevant, uncultured virus-host pairs. Viruses infecting Pelagibacterales, the predominant bacteria in surface oceans, have been studied through computational analyses and cultivation efforts. Here, we employ an accessible microfluidics and semi-permeable capsule (SPC) technology to investigate the uncultured pelagiphage vSAG 37-F6-host interactions since it is one of the most abundant and ubiquitous viruses in the marine virosphere. First, we validated this technology using cultured virus-host pairs. Then, marine single c

SUBMITTER: Martinez-Garcia M 

PROVIDER: S-EPMC12404659 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature