Selective IgM digestion improves anti-AAV IgG detection in the immune complex assay format.

ABSTRACT:

Aim

To confirm the observation of IgM interference in the anti-adeno-associated virus (AAV) IgG immune complex (IC) assay format and to verify that IgM-specific digestion can improve anti-AAV IgG detection in IC assays.

Methods

Treatment-emergent anti-AAV2 and anti-AAV9 IgG signals were measured in IC assays with and without IgM-specific digestion. Anti-AAV2 and anti-AAV9 IgM signals were measured in parallel.

Results

IgM digestion increased anti-AAV2 and anti-AAV9 IgG signals when anti-AAV2 or anti-AAV9 IgM were present in the matrix.

Conclusions

Co-existing anti-AAV IgM cause interference in the anti-AAV IC assay format. Selective IgM digestion improves the detection of anti-AAV IgG in the IC assay.

SUBMITTER: Wessels U 

PROVIDER: S-EPMC12416191 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature