Integrative analyses of single-cell and bulk RNA sequencing to construct the tumor-associated macrophage-related prognostic signature in lung adenocarcinoma.

ABSTRACT:

Background

Tumor-associated macrophages (TAMs) are a type of tumor-infiltrating immune cell that play a crucial role in tumor progression. However, the roles of TAMs and their regulatory mechanisms in lung adenocarcinoma (LUAD) remain poorly understood. Therefore, we aimed to develop a novel TAM-related prognostic signature to predict survival outcomes and constructed a lncRNA-miRNA-mRNA network based on these genes.

Methods

Transcriptomic data, clinical data, and single-cell RNA-sequencing (scRNA-seq) data were obtained from LUAD patients were obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Differentially expressed-lncRNAs (DE-lncRNAs), miRNAs (DE-miRNAs), and mRNAs (DEGs) were identified in LUAD. Differentially expressed TAM-relate

SUBMITTER: Yu C 

PROVIDER: S-EPMC12422256 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature