Screening of MYB1R1 interaction with <i>LDOX</i> promoter to regulate anthocyanin biosynthesis in peaches.

ABSTRACT:

Background

The floral color variegation of cultivar 'Sahong Tao' is distinctive and possesses significant ornamental value. Currently, there are no relevant reports on how MYB transcription factors (TFs) interact with LDOX promoter to regulate the flower color variegation in peach.

Methods

In this study, we screened for proteins that interact with the LDOX promoter using yeast one-hybrid (Y1H) and next-generation sequencing (NGS). The NGS data were aligned with the Arabidopsis database (TAIR10) utilizing Python 3.10.4. PlantTFDB was employed to identify TFs, while PlantRegMap was used to predict TFs that interact with the LDOX promoter. The Y1H assay verified MYB1R1 interaction with LDOX promoter, and Y1H-AOS predicted their binding sites.

SUBMITTER: Wu X 

PROVIDER: S-EPMC12422263 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature