Molecular Basis of Intron Retention in <i>PI-PLC γ1</i> mRNA from Osteoarthritis Synoviocytes.

ABSTRACT: Intron retention (IR) is one of the cellular mechanisms to perform alternative splicing and thus control gene expression in several mammalian cellular pathways. IR in PI-PLC γ1 mRNA was observed in some primary synoviocyte samples from osteoarthritis (OA) patients, likely due to inter-patient variability. The aim of the present manuscript was to explore the PI-PLC γ1 IR molecular mechanism as a consequence of nutraceutical treatment of synoviocytes and the molecular basis of individual response. To evaluate the gene expression modulation of molecules involved in mRNA splicing, an RNA-seq analysis was performed, and the transcription modulation of six differentially expressed genes was validated by RT-PCR. Moreover, through a silencing experiment, the relationship between P

SUBMITTER: Mariano A 

PROVIDER: S-EPMC12427997 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature