Allergens in Food: Analytical LC-MS/MS Method for the Qualitative Detection of <i>Pistacia vera</i>.

ABSTRACT: Pistachio (Pistacia vera) is widely consumed among tree nuts but capable of triggering severe IgE-mediated reactions in allergic individuals. Due to the similarity of cashew-borne and pistachio-borne allergen proteins and DNA, traditional detection methods, such as ELISA and PCR, often suffer from cross-reactivity, limiting their ability to discriminate between these two allergens. This study presents a sensitive LC-MS/MS method for the simultaneous detection of pistachio and cashew allergens in processed food with a screening detection limit (SDL) equal to 1 mg/kg. The method was validated for specificity, SDL, β error, precision, and ruggedness, and applied to various matrices (cereals, chocolate, sauces, and meat products). Ruggedness testing showed that all considered parameters

SUBMITTER: Giugliano R 

PROVIDER: S-EPMC12428185 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature