Knockdown of Telethonin Reduces Contractions and Provokes Aberrant Ca²⁺-waves in Human iPS Cell-induced Cardiomyocytes.

ABSTRACT:

Objectives

Dilated cardiomyopathy (DCM) is one of the leading causes of heart failure. To date, 48 genes are known to be associated with DCM. Telethonin, encoded by the TCAP gene, is a Z-disk protein that composes cytoskeletal structures and facilitates various signaling pathways in cardiomyocytes. At least, six TCAP variants have been found in patients with DCM. We sought to investigate the role of TCAP in cardiac function using TCAP-knockdown (KD) iPS cell (iPSC)-induced cardiomyocytes (CMs).

Methods & results

To investigate the role of TCAP in cardiomyocytes, the TCAP gene was knocked down in human iPS cells established from a healthy subject (201B7) using the CRISPR-Cas9 genome editing. The expressions of TCAP mRNA and tel

SUBMITTER: Handoh T 

PROVIDER: S-EPMC12441175 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature