ARIP1 Deficiency Facilitates the Inhibition of Neuronal Ferroptosis in Cerebral Ischemia by Activin A Through SMAD3 and p38 MAPK Signaling.

ABSTRACT:

Introduction

Ferroptosis is an essential pathophysiological process in cerebral ischemic injury. Activin receptor-interacting protein 1 (ARIP1) is a negative regulator of the activin signaling pathway in neurons.

Objective

This study investigated whether activin A inhibits neuronal ferroptosis and the role of ARIP1 in cerebral ischemic injury.

Methods and results

In this study, activin A increased the viability of primary neurons under conditions of oxygen-glucose deprivation (OGD). Subsequent RNA-sequencing analysis of activin A-treated neurons identified expression of Slc7a11 as the ferroptosis-associated gene with significant upregulation. Next, using the CRISPR/Cas9 system, mice were generated with a heterozygous deficiency of ARIP1 (Arip1^-/+), and the

SUBMITTER: Zou Z 

PROVIDER: S-EPMC12445200 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature