Detection of <i>Chlamydia trachomatis ompA</i> DNA in urine by loop-mediated isothermal amplification (LAMP) assay.

ABSTRACT:

Objectives

Efficient real-time PCR kits are commercially available for the detection of Chlamydia trachomatis (CT) genetic material, but at a price. As a result, cost-effective, sensitive and specific CT diagnostic tests are essential for resource limited countries. This study aims to describe the optimization of a loop mediated isothermal amplification (LAMP) assay for the detection of CT ompA DNA in urine.

Methods

Cost-saving modifications included using Bsm polymerase and a nucleic acid gel stain. Crude DNA extraction (method-1) involved centrifuging urine at 14,000 g for 30 min, heating the deposit at 95 °C for 5 min, and centrifuging again at 17,000 g for 1 min. To boost sensitivity, urinary inhibitors were diluted with phosphate-buffered sal

SUBMITTER: Attanayake H 

PROVIDER: S-EPMC12446907 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature