Evaluation of Inhibitory Activity of Novel Monoclonal Antibodies Against Cat Allergen Fel d 7 and Their Application to Analyse Allergen Extracts.

ABSTRACT: Current characterisation of cat dander/hair extracts used for allergy diagnosis or allergen-specific immunotherapy is mainly standardised towards the major allergen Fel d 1, while other allergens such as the lipocalin Fel d 7 are insufficiently characterised in such allergen products. This study aimed to produce recombinant Fel d 7 (rFel d 7) and murine IgG monoclonal antibodies (mAbs) specific to it for quantification in allergen extracts and assess the potential of the mAbs in inhibiting patients' IgE in functional assays. rFel d 7 was expressed in E. coli, purified by Ni-affinity and ion exchange chromatography, and physicochemically characterised by circular dichroism, Fourier-transform infrared spectroscopy, dynamic light scattering and mass spectrometry. Twenty hybridoma cell lines p

SUBMITTER: Rudokas V 

PROVIDER: S-EPMC12449780 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature