The Phenotype of <i>Physcomitrium patens SMC6</i> Mutant with Interrupted Hinge Interactions.

ABSTRACT: Background/Objectives: The Structural Maintenance of Chromosomes (SMC) proteins form essential heterocomplexes for the preservation of DNA structure and its functions, and hence cell viability. The SMC5/6 dimer is assembled by direct interactions of ATP heads via the kleisin NSE4 bridge and by SMC hinges. The structure might be interrupted by a single point mutation within a conserved motif of the SMC6-hinge. We describe the phenomena associated with the impairment of the SMC5/6 complex with morphology, repair of DNA double strand breaks (DSB), mutagenesis, recombination and gene targeting (GT) in the moss Physcomitrium patens (P. patens). Methods: Using CRISPR/Cas9-directed oligonucleotide replacement, we have introduced two close G to R point mutations in the

SUBMITTER: Angelis KJ 

PROVIDER: S-EPMC12469541 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature