Comparative Single-Cell Transcriptomic Landscape Reveals the Regulatory Mechanisms of Lactation during Selective Breeding in Asian Water Buffalo.

ABSTRACT: Characterizing the cell type-specific transcriptome is crucial for understanding the cellular and molecular regulatory mechanisms underlying adaptive evolution and complex phenotypes. Here, single-cell/nucleus RNA sequencing (sc/snRNA-seq) is used to construct a cell transcriptomic atlas of 397,011 cells, representing 57 cell types, from 12 tissues in river and swamp buffalo, which exhibit significant divergence in milk production. Differential expression analyses identify metabolic and secretory tissues (i.e., liver, mammary gland, and pituitary) and cell types (e.g., hepatocytes, luminal cells, somatotropes, and lactotropes) that mediate the divergence of milk production. Lactotrope-specific downregulation of TRHDE in river buffalo is associated with high milk production. Integrative ana

SUBMITTER: Dai D 

PROVIDER: S-EPMC12499429 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature