Differential Recognition of Lipopolysaccharide O-Antigens by the Pattern Recognition Molecules MBL and Ficolins of the Complement System.

ABSTRACT:

Introduction

The complement system plays a crucial role in bridging innate and adaptive immune responses. When activated, a proteolytic cascade leads to pathogen destruction. It is initiated via the recognition of foreign structures by three pathways: the classical, the lectin, and the alternative. This study focuses on the lectin pathway and the role of four pattern recognition molecules (PRMs), mannan-binding lectin, H-ficolin, L-ficolin, and M-ficolin, in the recognition of microbial patterns and the initiation of complement activation. These PRMs bind to specific carbohydrate structures; each PRM has unique ligand specificities. We investigated the PRM interactions with lipopolysaccharide (LPS) of Gram-negative bacteria.

Methods

Utilizing a microarray of 120 distinct LPS

SUBMITTER: Hymoller KM 

PROVIDER: S-EPMC12503535 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature