The significance of m6A-circFOXK2 in CD4⁺T cells from patients with rheumatoid arthritis and circFOXK2 correlates with Th17 % and autophagy.

ABSTRACT:

Objective

CD4⁺T cells are significant compositions of immune cells in rheumatoid arthritis (RA) and the aberrant function of CD4⁺T cells have been demonstrated to promote RA's progression. N6-methyladenosine (m6A) is a highly enriched modification found in CircRNAs. However, the role of m6A-methylated circRNA in regulation of CD4⁺T cells function in RA and its association with RA disease activity are presently unclear.

Methods

We used m6A-circRNA epitranscriptomic microarray analysis and m6A RNA immunocoprecipitation-quantitative polymerase chain reaction (MeRIP-qPCR) to screen and verify the differentially expressed m6A-methylated circRNAs in CD4⁺T cells from RA and HC.

Results

Many m6A-methylated circRNAs were differential

SUBMITTER: Luo Q 

PROVIDER: S-EPMC12554200 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature