Host Cell-Derived Extracellular Vesicles Regulate Iron Uptake in Recipient Macrophages during <i>Mycobacterium abscessus</i> Infection.

ABSTRACT: The role of host cell-derived extracellular vesicles (EVs) in host-pathogen interactions remains to be defined during mycobacterial infection. In this study, we characterized EVs from mouse RAW 264.7 cells uninfected or infected with Mycobacterium abscessus (M.ab), one of the most common nontuberculous mycobacterial (NTM) pathogens in humans. Our results show that M.ab infection increased the release of EVs but had no effect on the size distribution and total protein abundance of EVs from RAW 264.7 cells. Interestingly, EVs released by M.ab-infected RAW 264.7 cells significantly enhanced M.ab growth within recipient macrophages in cell culture. The proteomic analysis found that transferrin receptor was enriched in EVs from M

SUBMITTER: Ramos-Wolfley AD 

PROVIDER: S-EPMC12604032 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature