A Phosphoproteomic Analysis of Mycobacterial PknG-Mediated Host Immune Evasion.

ABSTRACT: Pathogenic mycobacteria, such as Mycobacterium tuberculosis, modulate the host immune system to evade clearance and promote long-term persistence, leading to disease progression or latent infection. Understanding how these mycobacteria evade elimination is key to uncovering the molecular mechanisms of infection. Protein kinase G (PknG) in pathogenic mycobacteria plays a critical role in avoiding macrophage clearance by inhibiting phagosome-lysosome fusion; however, the exact mechanism is not completely understood. To investigate the role of PknG during early events of macrophage infection, RAW 264.7 macrophages were infected with Mycobacterium bovis BCG wild-type and PknG knockout mutant strains. Phosphoproteomic analysis, including TiO₂-based phosphopeptide enrich

SUBMITTER: Baros-Steyl SS 

PROVIDER: S-EPMC12604046 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature