Single-cell multiomic modelling of early metastatic events promoted by the extracellular matrix.

ABSTRACT:

Background

Cancer metastasis, primarily driven by epithelial-to-mesenchymal transition (EMT), is responsible for most cancer-related mortalities. Traditional pre-clinical models fail to fully capture mesenchymal characteristics due to the loss of human stroma. The extracellular matrix (ECM) plays a crucial role in EMT, yet conventional in vitro models often rely on defined ECM components, which may not adequately replicate the human physiological ECM niche.

Methods

To mimic the in situ dissemination of cancer cells, we employed a patient-derived extracellular matrix (pdECM). We transitioned the culture matrix for patient-derived colorectal cancer organoids from a basement membrane extract (BME) to a patient-derived ECM (pdECM). We performed single-cell multiomic analyses, in

SUBMITTER: Lee HJ 

PROVIDER: S-EPMC12644670 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature