Extraction, Purification and Identification of Bovine Lung Peptides and Its Antioxidant Effects on H₂O₂-Induced HepG2 Cells and Mice with Alcoholic Liver Injury.

ABSTRACT: In this study, we constructed an extraction process for bovine lung peptide-1 (BLP-1) derived from bovine lung tissue utilizing single-factor optimization combined with response surface methodology. We systematically analyzed its antioxidant activity, biological safety, and therapeutic mechanisms against alcoholic liver disease (ALD). In vitro experiments demonstrated that BLP-1 exhibits excellent scavenging activity against various free radicals, while exhibiting no significant cytotoxicity or hemolytic activity. In a model of H₂O₂-induced oxidative damage in HepG2 cells, BLP-1 significantly alleviated oxidative stress injury by upregulating the activities of intracellular antioxidant enzymes. Animal experiments further confirmed that BLP-1 significantly reduced seru

SUBMITTER: Xiao X 

PROVIDER: S-EPMC12649525 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature